Journal: CNS Neuroscience & Therapeutics

Article Title: Cortical dopamine D5 receptors regulate neuronal circuit oscillatory activity and memory in rats

doi: 10.1111/cns.14210

Figure Lengend Snippet: Knockdown of the D5R in PFC increases GSK‐3β activity. (A) The experimental timeline is shown. (B) Electrode placements in the PFC, OFC, thalamus, and HIP. (C) Representative images and quantification of fluorescence showing reduced PFC expression of the D5R (top panels) and GSK‐3β phosphorylation at Ser9 (bottom panels) following D5R‐shRNA induced knockdown. N = 7 rats/group (D) Images showing drd5 gene expression in control (left panels) animals or following drd5 mRNA knockdown (right panels). (E) Quantification of the number of drd5 mRNA expressing cells. N = 3 rats/group, 2 slices/rat. Quantified data are expressed as percent control. ** p < 0.01, *** p < 0.001 Student's t test.

Article Snippet: Subsequently, adjacent slices were incubated in primary antibodies rabbit anti‐pGSK‐3β (Ser9) (catalogue #ab9107166, 1:200, Abcam) or rabbit anti‐D5R (catalogue #ADR‐005, 1:200, Alomone Labs) for 60 h at 4°C.

Techniques: Knockdown, Activity Assay, Fluorescence, Expressing, shRNA, Control

Journal: CNS Neuroscience & Therapeutics

Article Title: Cortical dopamine D5 receptors regulate neuronal circuit oscillatory activity and memory in rats

doi: 10.1111/cns.14210

Figure Lengend Snippet: Effect of PFC D5R knockdown on oscillatory power activity in rats. Effect of PFC D5R knockdown on oscillatory power activity in rats. (A–D) Power spectra (left and center panels) and quantification of power (right panels) are shown. PFC D5R knockdown had the following regional effects on spectral power, (A) increased theta power in PFC, (B) increased theta power in OFC, and (C) increased theta power in HIP. (D) No effects of PFC D5R knockdown were evident in spectral power in the thalamus. Power curves are presented as normalized data with jackknife estimates of SEM shown as shaded areas. Quantified data are expressed as percent control. N = 7–8 rats/group, 1–2 electrodes/region/rat. * p < 0.05 Student's t test.

Article Snippet: Subsequently, adjacent slices were incubated in primary antibodies rabbit anti‐pGSK‐3β (Ser9) (catalogue #ab9107166, 1:200, Abcam) or rabbit anti‐D5R (catalogue #ADR‐005, 1:200, Alomone Labs) for 60 h at 4°C.

Techniques: Knockdown, Activity Assay, Control

Journal: CNS Neuroscience & Therapeutics

Article Title: Cortical dopamine D5 receptors regulate neuronal circuit oscillatory activity and memory in rats

doi: 10.1111/cns.14210

Figure Lengend Snippet: Effect of PFC D5R knockdown on oscillatory coherence in rats. (A–F) Coherence spectra (left panels) and quantification (right panels) are shown. (A) PFC D5R knockdown increased PFC‐OFC theta coherence, and (B) reduced PFC–thalamus high gamma coherence. (C–F) No significant effects on coherence were observed between PFC–HIP, OFC‐HIP, OFC‐thalamus, or thalamus‐HIP. Coherence curves are presented with jackknife estimates of SEM shown as shaded areas. Quantified data are expressed as percent control. N = 7–8 rats/group, 1–2 electrodes/region/rat. * p < 0.05, ** p < 0.01 Student's t test.

Article Snippet: Subsequently, adjacent slices were incubated in primary antibodies rabbit anti‐pGSK‐3β (Ser9) (catalogue #ab9107166, 1:200, Abcam) or rabbit anti‐D5R (catalogue #ADR‐005, 1:200, Alomone Labs) for 60 h at 4°C.

Techniques: Knockdown, Control

Journal: CNS Neuroscience & Therapeutics

Article Title: Cortical dopamine D5 receptors regulate neuronal circuit oscillatory activity and memory in rats

doi: 10.1111/cns.14210

Figure Lengend Snippet: D5R knockdown in PFC induces deficits in learning and memory. (A) D5R knockdown in PFC induced deficits in object recognition memory in the NOR test. (B) Impaired spatial memory in the OL test was also evident. (C) D5R knockdown also induced impairment in associative recognition memory when tested in the OiP. (D) In the Y‐maze, D5R knockdown had no effects on short‐term recognition memory (left panel) but resulted in impairment on long‐term memory (right panel). N = 7–8 rats/group, * p < 0.05, ** p < 0.01, *** p < 0.001 Student's t test.

Article Snippet: Subsequently, adjacent slices were incubated in primary antibodies rabbit anti‐pGSK‐3β (Ser9) (catalogue #ab9107166, 1:200, Abcam) or rabbit anti‐D5R (catalogue #ADR‐005, 1:200, Alomone Labs) for 60 h at 4°C.

Techniques: Knockdown

Journal: Frontiers in Behavioral Neuroscience

Article Title: Strain-dependent regulation of hippocampal long-term potentiation by dopamine D1/D5 receptors in mice

doi: 10.3389/fnbeh.2022.1023361

Figure Lengend Snippet: Expression of D1 and D5 receptors are different in CaOlaHsd and C57Bl/6 mice. (A,B) The graphs depict the optical density of D1R (A) and D5R (B) expression in different layers of CA1 in CaOlaHsd (open black circles) and C57Bl/6 (closed gray circles) mice. D1 receptor expression is significantly higher in CaOlaHsd mice compared to C57Bl/6 mice in SLM (A) . The CA1 sublayers assessed show no differences with regard to D5 receptor expression in CaOlaHsd mice compared to C57Bl/6 mice (B) . SLM: stratum lacunosum moleculare, SO: stratum oriens, SP: stratum pyramidale, SR: stratum radiatum. (C,D) Immunostainings of the different layers in the CA1 region of CaOlaHsd and C57Bl/6 mice. The dotted magenta lines in (C) outline the stratum lacunosum moleculare, where D1R (C) showed increased expression in CaOlaHsd mice. No differences in expression of D5R were detected between strains (D) . Scale bar = 200 μm. Significant values are denoted by an asterisk. * p < 0.05.

Article Snippet: Subsequently, the sections were incubated overnight at room temperature with the primary antibody solution, which contained 20% biotin (avidin-biotin blocking kit, Vector Laboratories, Newark, CA, USA), 1% blocking serum, 0.2% Tx, and the primary antibody with these concentrations: D1R (1:100, rabbit polyclonal, ADR-005, Alomone Labs, Jerusalem, Israel), D5R (1:500, rabbit polyclonal, AB1765P, Sigma-Aldrich, Darmstadt, Germany).

Techniques: Expressing